Borrelia
Catalog number: BOR001Units | 200 µg |
Host | Rabbit |
Application |
ELISA Immunofluoresence Immunohistochemistry (paraffin) Western Blotting |
Background
The antibody is well suited to detect Borrelia in tissue slices and tissue extracts. Borrelia burgdorferi is a species of bacteria belonging to the class of spirochetes. Borrelia burgdorferi is the agent of Lyme Disease, a vector-borne disease transmitted by ticks (Ixodes ricinus and Ixodes dammini). Borrelia is widely distributed in North America and Europe. Borrelia burgdorferi, cross-reactivity with Borrelia hermsii and Borrelia parkerii as well as Treponema pallidum. In Western blotting bands are seen at 83kDa, 41kDa, 34kDa, 31kDa. Additional lower MW bands may also be observed. .
Source
Immunogen: Cell preparation of Borrelia burgdorferi
Product
Purified IgG in PBS pH7.2, contains sodium azide (0,09%)**
Purification Method: Purified IgG in PBS pH7.2, contains sodium azide (0,09%)**
Secondary Reagents: Anti-Rabbit IgG:Biotin-conjugate (Art. No. ZU101) in combination with Streptavidin:HRPO (Art. No. ZU054) or Streptavidin:AP (Art. No. ZU051)
Concentration: 1 mg/ml
Specificity
Species Reactivity: Borrelia, (Treponema)
Applications
IHC(P), WB, IFA, ELISA
Incubation Time: 60 min at RT or 18 hr at 2-8°C
Working Concentration: (liquid conc.) WB: 1 µg/ml, IHC: 5 µg/ml
Storage
2-8°C
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
References
1. Buechner S A , Winkelmann R K, Lautenschlager S ,Gilli L , Rufli T (1993) Localized scleroderma associated with Borrelia burgdorferi infection. Clinical, histologic, and immunohistochemical observations Journal of the American Academy of Dermatology. 29(2 Pt 1):190-6.
2. Quatresooz P., Piérard G. E. (2009) Skin Homing of Treponema pallidum in Early Syphilis: An Immunohistochemical Study Applied Immunohistochemistry & Molecular Morphology: Volume 17 - Issue 1 - pp 47-50
3. Arrese Estrada J; Mélotte P; Hermanns J F; Piérard G E (1991) Immunohistochemistry of Borrelia type spirochetes Annales de dermatologie et de vénéréologie 118(4):277-9.
Safety Datasheet(s) for this product:
NM_Sodium Azide |