Catalogue
A431 cells stimulated with EGF
Catalog number: X1003Product Type |
Control Lysate |
Units | 100 µl |
Application |
Western Blotting |
Background
A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.
Synonyms: A431 stimulated with EGF control cell lysate
Product
Product Form: Unconjugated
Formulation: Provided as solution in Laemmli electrophoresis sample buffer. Samples need to be boiled for 3-5 minutes, before loading the gel.
Concentration: See vial for concentration
Applications
Suggested use: A volume of 10-25ul is suggested for immunobloting.
Functional Analysis: Western Blotting
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Room Temperature
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.