Chicken anti Human placenta-derived cytonectin (not affinity purified)
Catalog number: AHC| Product Type |
Primary antibodies |
| Units | 10 mg |
| Host | Chicken |
| Application |
ELISA Western Blotting |
Source
Cytonectin was isolated from a cell lysate of normal human third trimester placenta by reversed-order OFarrell gel electrophoresis (ROOGE). Purified cytonectin was used to immunize a chicken. After multiple immunizations, eggs were collected and IgY was purified from the egg yolk.
Product
Product Form: Egg yolk-derived purified IgY
Formulation: Phosphate buffered saline‚ pH 7.2 with 0.05% sodium azide
Purification Method: Egg yolk-derived purified IgY (non affinity purified)
Concentration: See vial for concentration.
Applications
For immunoblotting, antibody is diluted to a 1 mg/ml stock solution. This is diluted 1:1000 immediately prior to blot application. The bound primary antibody is detected by HRP-goat anti-chicken IgY Fc fragment as secondary antibody, followed by any chemiluminescence protocol. The anti-cytonectin antibody is also useful for immunomicroscopy. Most tissues have some cytonectin detectable. Many pathologic tissues, especially neoplastic cells, show increased cytonectin expression. The antigens are highly evolutionarily conserved in human, rodent, and avian tissues.
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles.
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Safety Datasheet(s) for this product:
| NM_Sodium Azide |