Chicken anti Green Fluorescent Protein (GFP) [Neisseria gonorrhoeae]
Catalog number: AGFP| Product Type |
Primary antibodies |
| Units | 10 mg |
| Host | Chicken |
| Application |
ELISA Western Blotting |
Source
Chickens were immunized with recombinant full-length bacterial gonococci Green Fluorescent Protein (GFP) [Neisseria gonorrhoeae] expressed in E. coli cells. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified.
Product
Product Form: Egg yolk-derived purified IgY
Formulation: Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide
Purification Method: Egg yolk-derived purified IgY (non affinity purified)
Concentration: See vial for concentration.
Applications
In a Western blot, the anti-GFP used at between 1:3000 and 1:5000 dilution could detect 10 ng of GFP in an A549 cell lysate (see image). Optimal conjugate working dilutions should be determined for your particular assay conditions.
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles.
Shipping Conditions: Ship at ambient temperature.
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Safety Datasheet(s) for this product:
| NM_Sodium Azide |