Chicken anti Clostridium difficile Binary Toxin, Subunit A
Catalog number: ACdBTSA| Product Type |
Primary antibodies |
| Units | 0.1 mg |
| Host | Chicken |
| Species Reactivity |
Clostridium |
| Application |
ELISA Western Blotting |
Source
Chickens were immunized with highly purified Binary Toxin, A subunit, purified from Clostridium difficile. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Binary Toxin, Subunit A was immunoaffinity purified using the immunogen immobilized on a solid support.
Product
Product Form: Affinity purified
Formulation: Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide
Purification Method: Egg-yolk derived IgY Affinity Purified
Concentration: See vial for concentration.
Applications
This antibody has been tested in direct ELISA and Western blot. A 1:4000 dilution was an effective probe for 125 ng of C. difficile Binary Toxin A Subunit. The antibody titre, measured at the midpoint, was 1:56,000 of a 2.0 mg/ml solution (~90 ng/ml) using a 2 ug coating of C. difficile Binary Toxin, A Subunit. Optimal working dilutions should be determined for your particular assay conditions.
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles.
Shipping Conditions: Ship at ambient temperature
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Safety Datasheet(s) for this product:
| NM_Sodium Azide |