Catalogue

Background
The reactivity of the antiserum is directed to horseradish peroxidase as tested in direct binding enzyme immunoassay, ELISA and immunoblotting. As a control in the tests where mouse IgG1 (with irrelevant specificity) should be used to show the presence of that given mouse isotype. In indirect tests as reagent to horseradish peroxidase or to potentiate the visualisation of a peroxidase labelled conjugate in the appropriate material. As secondary reagent a labelled anti mouse IgG will serve for the detection. This system can be further be potentiated by the use of anti-FITC or anti-TRITC conjugates. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Source
immunogen: Highly purified horseradish peroxidase.

Product
Delipidated, heat inactivated, lyophilized, stable whole ascites. IgG concentration is 1.0 mg/ml. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water.

Formulation: Delipidated, heat inactivated, lyophilized, stable whole ascites. IgG concentration is 1.0 mg/ml. No foreign proteins added.

Specificity
Monoclonal Mouse antibody to Horseradish peroxidase

Species Reactivity: This product has not been tested for cross-reactivity.

Applications
Indirect immunofluorescence,ELISA.

Storage
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.